Hotspot

trnH-GUG-psbA

LSC · 62-400 · 339 bp

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Pi 0.051
MarkerSeek score 74.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
339
variable_sites
173
pi
0.051
unique_haplotype_count
64
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA TAGACCTAGCCGCTCTTGAA 50.0 50.0 57.9 57.9 238-575 bp 1.0 89.8
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC TAGACCTAGCCGCTCTTGAA 50.0 50.0 57.9 57.9 237-574 bp 1.0 89.8
trnH-GUG-psbA_p3 ACAATCCACTGCCTTGATCC TAGACCTAGCCGCTCTTGAA 50.0 50.0 57.9 57.9 239-576 bp 1.0 89.8
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA CTAGACCTAGCCGCTCTTGA 50.0 55.0 57.9 58.3 239-576 bp 1.0 88.4
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CTAGACCTAGCCGCTCTTGA 50.0 55.0 57.9 58.3 238-575 bp 1.0 88.4