Hotspot

ycf1

SSC · 106765-112242 · 5478 bp

Back to Lomatogonium

Pi 0.358

MarkerSeek score 75.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 1718
pi: 0.358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTGTCCCGTGTCAACTCTA	CAACGTTTCAGCCTGGGATA	50.0	50.0	58.0	57.9	1051 bp	0.5	59.1
ycf1_p2	AGTGTCCCGTGTCAACTCTA	AACGTTTCAGCCTGGGATAC	50.0	50.0	58.0	57.9	1050 bp	0.5	59.1
ycf1_p3	AAAGTGTCCCGTGTCAACTC	CAACGTTTCAGCCTGGGATA	50.0	50.0	58.1	57.9	1053 bp	0.5	58.7
ycf1_p4	AAAGTGTCCCGTGTCAACTC	AACGTTTCAGCCTGGGATAC	50.0	50.0	58.1	57.9	1052 bp	0.5	58.7
ycf1_p5	AGTGTCCCGTGTCAACTCTA	AGCTGCTCGCCTTGTTAAAT	50.0	45.0	58.0	58.2	861 bp	0.5	58.5