Hotspot

ndhF

SSC · 123856-126093 · 2238 bp

Back to Lomatogonium

Pi 0.468

MarkerSeek score 80.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 933
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCGCATATCTTCCCTTTTGC	GCAGAATACCGTTACCCGTT	50.0	50.0	57.9	58.0	2579 bp	0.5	58.9
ndhF_p2	GCGCATATCTTCCCTTTTGC	ATCCGGGCAAAATCGATGAA	50.0	45.0	57.9	57.9	2501 bp	0.5	58.6
ndhF_p3	GCGCATATCTTCCCTTTTGC	CCGGGCAAAATCGATGAAAC	50.0	50.0	57.9	58.1	2499 bp	0.5	58.3
ndhF_p4	GCGCATATCTTCCCTTTTGC	GGGCAAAATCGATGAAACGG	50.0	50.0	57.9	58.1	2497 bp	0.5	58.3
ndhF_p5	GCGCATATCTTCCCTTTTGC	TCCGGGCAAAATCGATGAAA	50.0	45.0	57.9	58.2	2500 bp	0.5	57.6