Hotspot

ccsA

SSC · 120812-121804 · 993 bp

Back to Lomatogonium

Pi 0.468

MarkerSeek score 81.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 993
variable_sites: 408
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ATGAGTGGCTTTGTTGCTGA	AAGTTCAACACAAGCCGCTA	45.0	45.0	58.0	58.0	1733 bp	0.5	59.6
ccsA_p2	ATGAGTGGCTTTGTTGCTGA	TAAGTTCAACACAAGCCGCT	45.0	45.0	58.0	58.0	1734 bp	0.5	59.6
ccsA_p3	TACGATTCGTGTCGGTTCAC	AAGTTCAACACAAGCCGCTA	50.0	45.0	58.0	58.0	1246 bp	0.5	59.4
ccsA_p4	TACGATTCGTGTCGGTTCAC	TAAGTTCAACACAAGCCGCT	50.0	45.0	58.0	58.0	1247 bp	0.5	59.4
ccsA_p5	GCTTGCATTACCGGGTATGA	AAGTTCAACACAAGCCGCTA	50.0	45.0	58.0	58.0	1749 bp	0.5	59.4