Hotspot

trnL-UAG-ndhF

SSC · 121971-123855 · 1885 bp

Back to Lomatogonium

Pi 0.46

MarkerSeek score 78.10

Primer available yes

Label trnL-UAG-ndhF

Feature Metadata

label_name: trnL-UAG-ndhF
feature_type: igs
region: SSC
length_bp: 1885
variable_sites: 680
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ndhF_p1	TAGCTAGCAGGCCTTTTTCG	CAAGAGGTCGTGTGAACCAA	50.0	50.0	58.0	58.1	2716 bp	0.5	59.4
trnL-UAG-ndhF_p2	TCGAACCGAGATGCTTAAGC	CAAGAGGTCGTGTGAACCAA	50.0	50.0	58.1	58.1	2461 bp	0.5	59.0
trnL-UAG-ndhF_p3	AACCACTCGGATTGAAGAGC	CAAGAGGTCGTGTGAACCAA	50.0	50.0	57.9	58.1	2981 bp	0.5	58.8
trnL-UAG-ndhF_p4	AAGCACTGCTTCCTAAGAGC	CAAGAGGTCGTGTGAACCAA	50.0	50.0	57.9	58.1	2445 bp	0.5	58.7
trnL-UAG-ndhF_p5	TCCTAAGAGCAGCGTGTCTA	CAAGAGGTCGTGTGAACCAA	50.0	50.0	57.9	58.1	2435 bp	0.5	58.6