Hotspot

psaC-ndhD

SSC · 118962-119431 · 470 bp

Back to Lomatogonium

Pi 0.494

MarkerSeek score 78.60

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 470
variable_sites: 201
pi: 0.494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	AACAGAAGACTGCGTTGGTT	TCTTTCCTCCCCACATGGAT	45.0	50.0	58.0	58.0	709 bp	0.5	59.6
psaC-ndhD_p2	AACAGAAGACTGCGTTGGTT	TAATCGGCGATTTGACAGCA	45.0	45.0	58.0	58.0	916 bp	0.5	59.6
psaC-ndhD_p3	AACAGAAGACTGCGTTGGTT	ACCCATACACAGAACTCCCA	45.0	50.0	58.0	58.0	792 bp	0.5	59.4
psaC-ndhD_p4	AACAGAAGACTGCGTTGGTT	ATACGAACCAACCCATACGC	45.0	50.0	58.0	58.1	1025 bp	0.5	59.2
psaC-ndhD_p5	AACAGAAGACTGCGTTGGTT	GCCGAGTACAGACGTTTCTT	45.0	50.0	58.0	57.9	725 bp	0.5	59.2