Hotspot

ndhE-psaC

SSC · 118391-118715 · 325 bp

Back to Lomatogonium

Pi 0.401

MarkerSeek score 76.90

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 325
variable_sites: 125
pi: 0.401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	ACCGGCTATTGTTTCAGCAA	CCAGAAAAACCAGTGCTCCT	45.0	50.0	58.1	58.0	743 bp	0.5	59.1
ndhE-psaC_p2	TGGACCGGCTATTGTTTCAG	CCAGAAAAACCAGTGCTCCT	50.0	50.0	57.9	58.0	746 bp	0.5	59.0
ndhE-psaC_p3	ACCGGCTATTGTTTCAGCAA	AACCAACGCAGTCTTCTGTT	45.0	45.0	58.1	58.0	552 bp	0.5	58.9
ndhE-psaC_p4	AGTTGCTTTAATAGGCGCCA	CCAGAAAAACCAGTGCTCCT	45.0	50.0	57.9	58.0	1131 bp	0.5	58.9
ndhE-psaC_p5	ACCGGCTATTGTTTCAGCAA	GCCAAGCCGATAAAGTAGCT	45.0	50.0	58.1	58.0	1082 bp	0.5	58.9