Hotspot

ndhI-ndhG

SSC · 117041-117435 · 395 bp

Back to Lomatogonium

Pi 0.398

MarkerSeek score 77.00

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 395
variable_sites: 136
pi: 0.398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	CAACCTCGCCTCAACTTCTT	CAATCCCAGCGAAAAAGCAG	50.0	50.0	58.1	58.0	1127 bp	0.5	59.0
ndhI-ndhG_p2	CAACCTCGCCTCAACTTCTT	TGACCCCTAAACCCCCTAAG	50.0	55.0	58.1	58.0	1083 bp	0.5	58.9
ndhI-ndhG_p3	CAACCTCGCCTCAACTTCTT	AAATCCGGTATTGGTTCGGG	50.0	50.0	58.1	58.0	1033 bp	0.5	58.7
ndhI-ndhG_p4	ATGGACTTTACCAAGGCTGC	CAATCCCAGCGAAAAAGCAG	50.0	50.0	58.2	58.0	1216 bp	0.5	58.6
ndhI-ndhG_p5	CCTCGCCTCAACTTCTTTCA	CAATCCCAGCGAAAAAGCAG	50.0	50.0	57.8	58.0	1124 bp	0.5	58.5