Hotspot

petA-psbJ

LSC · 61660-62522 · 863 bp

Back to Lomatogonium

Pi 0.0457

MarkerSeek score 79.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 39
pi: 0.0457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCATTACGTGTCCAAGGAC	CATATTCCGGATTGGGGTCA	55.0	50.0	58.3	57.1	1010 bp	1.0	74.1
petA-psbJ_p2	GACCCATTACGTGTCCAAGG	CATATTCCGGATTGGGGTCA	55.0	50.0	58.3	57.1	1012 bp	1.0	74.1
petA-psbJ_p3	ACGTGTCCAAGGACTTTTGT	CATATTCCGGATTGGGGTCA	45.0	50.0	57.6	57.1	1004 bp	1.0	72.9
petA-psbJ_p4	CCCATTACGTGTCCAAGGAC	GCTGGTATTCTTGTGATCGGT	55.0	47.6	58.3	58.1	1056 bp	1.0	72.8
petA-psbJ_p5	GACCCATTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	58.3	58.1	1058 bp	1.0	72.8