Hotspot

psbB

LSC · 68348-69874 · 1527 bp

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Pi 0.00196
MarkerSeek score 59.80
Primer available yes
Label psbB

Feature Metadata

label_name
psbB
feature_type
gene
region
LSC
length_bp
1527
variable_sites
3
pi
0.00196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB_p1 TGGATAGTCTTTTCCAATGCGA GGGAGCTGTTTTGTTTCCCA 40.9 50.0 57.8 58.6 1664 bp 1.0 57.7
psbB_p2 TGGATAGTCTTTTCCAATGCGA TGGGAGCTGTTTTGTTTCCC 40.9 50.0 57.8 58.6 1665 bp 1.0 57.7
psbB_p3 TGGATAGTCTTTTCCAATGCGA AAGGATGGGAGCTGTTTTGT 40.9 45.0 57.8 57.3 1670 bp 1.0 57.0
psbB_p4 TGGATAGTCTTTTCCAATGCGA AGGATGGGAGCTGTTTTGTT 40.9 45.0 57.8 57.3 1669 bp 1.0 57.0
psbB_p5 TGGATAGTCTTTTCCAATGCGA GGAGCTGTTTTGTTTCCCATG 40.9 47.6 57.8 58.0 1663 bp 1.0 54.2