Hotspot

ndhJ

LSC · 47920-48399 · 480 bp

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Pi 0.0187
MarkerSeek score 56.50
Primer available yes
Label ndhJ

Feature Metadata

label_name
ndhJ
feature_type
gene
region
LSC
length_bp
480
variable_sites
9
pi
0.0187
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ_p1 CGTTTCATCTAGGAACAGAACG TCTTGCATCCGAAATGTGAA 45.5 40.0 57.2 55.7 622 bp 1.0 41.2
ndhJ_p2 CGTTTCATCTAGGAACAGAACG TTCTTGCATCCGAAATGTGA 45.5 40.0 57.2 55.7 623 bp 1.0 41.2
ndhJ_p3 TCCGTTTCATCTAGGAACAGA TCTTGCATCCGAAATGTGAA 42.9 40.0 56.1 55.7 624 bp 1.0 41.2
ndhJ_p4 TCCGTTTCATCTAGGAACAGA TTCTTGCATCCGAAATGTGA 42.9 40.0 56.1 55.7 625 bp 1.0 41.2
ndhJ_p5 CGTTTCATCTAGGAACAGAACG CTTTCTTGCATCCGAAATGTGA 45.5 40.9 57.2 57.6 625 bp 1.0 41.2