Hotspot

rpl32-trnL-UAG

SSC · 104547-105202 · 656 bp

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Pi 0.0259
MarkerSeek score 73.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
656
variable_sites
17
pi
0.0259
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAATGAGCATCCAAAACCA TTCGAACCGAGATGCTTGAG 45.0 50.0 57.3 58.0 775-781 bp 1.0 75.7
rpl32-trnL-UAG_p2 GCAATGAGCATCCAAAACCA GGATTCGAACCGAGATGCTT 45.0 50.0 57.3 58.1 778-784 bp 1.0 75.3
rpl32-trnL-UAG_p3 GCAATGAGCATCCAAAACCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.3 57.9 748-754 bp 1.0 74.8
rpl32-trnL-UAG_p4 GCAATGAGCATCCAAAACCA AGCAGCGTGTCTACCAATTT 45.0 45.0 57.3 57.8 741-747 bp 1.0 74.4
rpl32-trnL-UAG_p5 ATTTTCGAGTGGCAATGAGC TTCGAACCGAGATGCTTGAG 45.0 50.0 57.1 58.0 786-792 bp 1.0 74.3