Hotspot

clpP-psbB

LSC · 67811-68347 · 537 bp

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Pi 0.00372
MarkerSeek score 59.60
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
537
variable_sites
2
pi
0.00372
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 ACCCAAGTCGCTTCTTCATC TATAAAGCCATCGAGCCAGC 50.0 50.0 57.9 57.8 718 bp 1.0 78.1
clpP-psbB_p2 ACCCAAGTCGCTTCTTCATC CCAGCCCAACCAGAAACTAG 50.0 55.0 57.9 58.2 703 bp 1.0 78.0
clpP-psbB_p3 TCAACCCAAGTCGCTTCTTC TATAAAGCCATCGAGCCAGC 50.0 50.0 58.1 57.8 721 bp 1.0 77.9
clpP-psbB_p4 TCAACCCAAGTCGCTTCTTC CCAGCCCAACCAGAAACTAG 50.0 55.0 58.1 58.2 706 bp 1.0 77.9
clpP-psbB_p5 ACCCAAGTCGCTTCTTCATC CGATACCAAGGCAAACCCAT 50.0 50.0 57.9 58.2 619 bp 1.0 77.6