Hotspot

atpI-atpH

LSC · 31097-31665 · 569 bp

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Pi 0.00351
MarkerSeek score 60.10
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
569
variable_sites
2
pi
0.00351
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TTTTGCAACATTAGCCGCAG GCAATAACAGAAGCAGCAGC 45.0 50.0 57.9 58.1 659 bp 1.0 79.0
atpI-atpH_p2 TTTTGCAACATTAGCCGCAG GCAGCAATAACAGAAGCAGC 45.0 50.0 57.9 58.1 662 bp 1.0 79.0
atpI-atpH_p3 TTTTGCAACATTAGCCGCAG GCAGTACCTTGACCAACTCC 45.0 55.0 57.9 58.2 716 bp 1.0 78.2
atpI-atpH_p4 TTTTGCAACATTAGCCGCAG AAGCAGCAGCAATTAGTGGA 45.0 45.0 57.9 57.8 649 bp 1.0 78.1
atpI-atpH_p5 TTTTGCAACATTAGCCGCAG GCCAATCCAGCAGCAATAAC 45.0 50.0 57.9 57.8 671 bp 1.0 78.0