Hotspot

matK

LSC · 2226-3752 · 1527 bp

Back to Loeseneriella

Pi 0.00593

MarkerSeek score 62.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 9
pi: 0.00593
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACTCCGTTTTTGAACCCGTA	TGCTTTGACTGTATCGCACT	45.0	45.0	57.4	57.5	1695-1704 bp	1.0	72.3
matK_p2	TACTCCGTTTTTGAACCCGT	TGCTTTGACTGTATCGCACT	45.0	45.0	57.4	57.5	1696-1705 bp	1.0	72.3
matK_p3	TCCGTTTTTGAACCCGTACT	TGCTTTGACTGTATCGCACT	45.0	45.0	57.4	57.5	1693-1702 bp	1.0	72.3
matK_p4	ACTCCGTTTTTGAACCCGTA	ACCTTTTCCCTTCGGTTCAA	45.0	45.0	57.4	57.3	1646-1655 bp	1.0	70.0
matK_p5	TCCGTTTTTGAACCCGTACT	ACCTTTTCCCTTCGGTTCAA	45.0	45.0	57.4	57.3	1644-1653 bp	1.0	70.0