Hotspot

ccsA-ndhD

SSC · 118032-118288 · 257 bp

Back to Loeseneriella

Pi 0.0251

MarkerSeek score 63.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 257
variable_sites: 6
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAGGAATAGGGCTGCACAGT	GGCATTTATCCGGACTTCGT	50.0	50.0	58.1	58.1	360-376 bp	1.0	83.8
ccsA-ndhD_p2	TAGGAATAGGGCTGCACAGT	TGGCATTTATCCGGACTTCG	50.0	50.0	58.1	58.1	361-377 bp	1.0	83.8
ccsA-ndhD_p3	GGCTGCACAGTTATGGTTCA	GGCATTTATCCGGACTTCGT	50.0	50.0	58.5	58.1	351-367 bp	1.0	81.2
ccsA-ndhD_p4	GGCTGCACAGTTATGGTTCA	TGGCATTTATCCGGACTTCG	50.0	50.0	58.5	58.1	352-368 bp	1.0	81.2
ccsA-ndhD_p5	ATAGGGCTGCACAGTTATGG	GGCATTTATCCGGACTTCGT	50.0	50.0	57.4	58.1	355-371 bp	1.0	79.7