Hotspot

trnN-GUU-ndhF

IRb · 111687-113788 · 2102 bp

Back to Loeseneriella

Pi 0.0138

MarkerSeek score 53.50

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2102
variable_sites: 29
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AAGACAGAGGGAACGGGTTA	CTTGTTACACGGCCGGATTA	50.0	50.0	58.0	58.0	2446-2605 bp	1.0	80.8
trnN-GUU-ndhF_p2	ATCTCCCCAGGTAGGATTCG	CTTGTTACACGGCCGGATTA	55.0	50.0	58.0	58.0	2364-2523 bp	1.0	80.7
trnN-GUU-ndhF_p3	CGGGAGAATCAATGACTGCA	CTTGTTACACGGCCGGATTA	50.0	50.0	58.0	58.0	2644-2803 bp	1.0	80.7
trnN-GUU-ndhF_p4	GGCAGAATAGCAGAGCAAGT	CTTGTTACACGGCCGGATTA	50.0	50.0	58.0	58.0	2720-2879 bp	1.0	80.6
trnN-GUU-ndhF_p5	ATCTCCCCAGGTAGGATTCG	GCTTTAGGTCTGGGCTCTTC	55.0	55.0	58.0	58.0	2671-2830 bp	1.0	80.5