Hotspot

petL-petG

LSC · 68010-68180 · 171 bp

Back to Loeseneriella

Pi 0.0175

MarkerSeek score 62.60

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 3
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TTCGGTTTTCTACTAGCGGC	TCAAAGGTCCAGCTGATCAC	50.0	50.0	58.0	57.5	360 bp	1.0	76.8
petL-petG_p2	CGGTTTTCTACTAGCGGCTT	TCAAAGGTCCAGCTGATCAC	50.0	50.0	58.0	57.5	358 bp	1.0	76.8
petL-petG_p3	TTCGGTTTTCTACTAGCGGC	CAAAGGTCCAGCTGATCACC	50.0	55.0	58.0	58.5	359 bp	1.0	76.3
petL-petG_p4	CGGTTTTCTACTAGCGGCTT	CAAAGGTCCAGCTGATCACC	50.0	55.0	58.0	58.5	357 bp	1.0	76.3
petL-petG_p5	TTCGGTTTTCTACTAGCGGC	AGCTGATCACCCCGTCTATA	50.0	50.0	58.0	57.3	350 bp	1.0	75.3