Hotspot

petA-psbJ

LSC · 64964-66020 · 1057 bp

Back to Loeseneriella

Pi 0.0246

MarkerSeek score 72.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1057
variable_sites: 26
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACCCAGTACGTGTACAAGGT	ATTCTGGATTGGGCTCATCC	50.0	50.0	58.3	57.3	1202-1237 bp	1.0	74.0
petA-psbJ_p2	CCCAGTACGTGTACAAGGTC	ATTCTGGATTGGGCTCATCC	55.0	50.0	57.6	57.3	1201-1236 bp	1.0	73.5
petA-psbJ_p3	GACCCAGTACGTGTACAAGG	ATTCTGGATTGGGCTCATCC	55.0	50.0	57.6	57.3	1203-1238 bp	1.0	73.5
petA-psbJ_p4	CGCATCAGTTATTTTGGCACA	ATTCTGGATTGGGCTCATCC	42.9	50.0	57.8	57.3	1168-1203 bp	1.0	66.7
petA-psbJ_p5	CCAGTACGTGTACAAGGTCT	ATTCTGGATTGGGCTCATCC	50.0	50.0	56.6	57.3	1200-1235 bp	1.0	65.2