Hotspot

psaI-ycf4

LSC · 61481-61951 · 471 bp

Back to Loeseneriella

Pi 0.0193

MarkerSeek score 67.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 9
pi: 0.0193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GGCAATTGCAATGGCTTCTT	CGTTCTGACCGACAACTCAT	45.0	50.0	57.9	57.9	542-546 bp	1.0	80.6
psaI-ycf4_p2	GTTGTTCCGGCAATTGCAAT	CGTTCTGACCGACAACTCAT	45.0	50.0	58.2	57.9	550-554 bp	1.0	79.7
psaI-ycf4_p3	TTGTTCCGGCAATTGCAATG	CGTTCTGACCGACAACTCAT	45.0	50.0	58.2	57.9	549-553 bp	1.0	79.7
psaI-ycf4_p4	CTAGTTGTTCCGGCAATTGC	CGTTCTGACCGACAACTCAT	50.0	50.0	57.7	57.9	553-557 bp	1.0	79.2
psaI-ycf4_p5	TAGTTGTTCCGGCAATTGCA	CGTTCTGACCGACAACTCAT	45.0	50.0	58.4	57.9	552-556 bp	1.0	78.2