Hotspot

trnM-CAU-atpE

LSC · 54760-55013 · 254 bp

Back to Loeseneriella

Pi 0.0329

MarkerSeek score 64.30

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 8
pi: 0.0329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	353-371 bp	1.0	80.7
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	50.0	55.0	58.4	58.3	352-370 bp	1.0	78.8
trnM-CAU-atpE_p3	GTATTGCTTTCATACGGCGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.3	58.0	354-372 bp	1.0	78.6
trnM-CAU-atpE_p4	GCGGGAGTCATTGGTTCAAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.8	58.0	338-356 bp	1.0	78.1
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	GGAGGCTATCAATGGGATGG	50.0	55.0	58.4	57.5	328-346 bp	1.0	77.2