Hotspot

psbC-trnS-UGA

LSC · 36641-36880 · 240 bp

Back to Loeseneriella

Pi 0.0292

MarkerSeek score 69.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 7
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TTGATCGCGATTTTGAGCCT	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.3	58.1	364 bp	1.0	79.0
psbC-trnS-UGA_p2	GCTGCGGGATTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.3	58.1	386 bp	1.0	78.7
psbC-trnS-UGA_p3	TTGATCGCGATTTTGAGCCT	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.3	58.1	375 bp	1.0	78.4
psbC-trnS-UGA_p4	GCTGCGGGATTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.3	58.1	397 bp	1.0	78.1
psbC-trnS-UGA_p5	TTGATCGCGATTTTGAGCCT	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.3	58.2	310 bp	1.0	78.0