Hotspot

psbM-trnD-GUC

LSC · 30193-31318 · 1126 bp

Back to Loeseneriella

Pi 0.00631

MarkerSeek score 63.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1126
variable_sites: 7
pi: 0.00631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	1224-1234 bp	1.0	75.6
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1223-1233 bp	1.0	74.7
psbM-trnD-GUC_p3	AATGAACAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	1225-1235 bp	1.0	73.9
psbM-trnD-GUC_p4	AGAATGAACAGCGCAGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.6	58.5	1227-1237 bp	1.0	72.6
psbM-trnD-GUC_p5	GCCCTGACTTTGACTGATCG	TTCAATTGGTCAGAGCACCG	55.0	50.0	58.6	58.5	1285-1295 bp	1.0	72.5