Hotspot

trnS-GCU-trnG-UCC

LSC · 7917-8748 · 832 bp

Back to Loeseneriella

Pi 0.0185
MarkerSeek score 68.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
832
variable_sites
15
pi
0.0185
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TATGAATCCAACCGAGGGAC 50.0 50.0 58.0 56.7 948-972 bp 1.0 72.5
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TATGAATCCAACCGAGGGAC 50.0 50.0 58.0 56.7 947-971 bp 1.0 72.4
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ATGAATCCAACCGAGGGACC 50.0 55.0 58.0 59.5 947-971 bp 1.0 71.2
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ATGAATCCAACCGAGGGACC 50.0 55.0 58.0 59.5 946-970 bp 1.0 71.2
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC TATGAATCCAACCGAGGGAC 50.0 50.0 58.4 56.7 950-974 bp 1.0 69.6