Hotspot

rps16-trnQ-UUG

LSC · 5397-6423 · 1027 bp

Back to Loeseneriella

Pi 0.0111

MarkerSeek score 57.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1027
variable_sites: 11
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCGTTGTTTTCGACCACACT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1067-1093 bp	1.0	82.8
rps16-trnQ-UUG_p2	CGTTGTTTTCGACCACACTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1066-1092 bp	1.0	82.2
rps16-trnQ-UUG_p3	TCGTTGTTTTCGACCACACT	AATAGAATATGGGGCGTGGC	45.0	50.0	58.0	57.8	1124-1150 bp	1.0	81.6
rps16-trnQ-UUG_p4	GACCACACTGTCTCAAACGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1056-1082 bp	1.0	81.2
rps16-trnQ-UUG_p5	CGTTGTTTTCGACCACACTG	AATAGAATATGGGGCGTGGC	50.0	50.0	57.9	57.8	1123-1149 bp	1.0	81.0