Hotspot

trnH-GUG-psbA

LSC · 67-628 · 562 bp

Back to Loeseneriella

Pi 0.0116
MarkerSeek score 68.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
562
variable_sites
6
pi
0.0116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 664-679 bp 1.0 83.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 678-693 bp 1.0 83.4
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 677-692 bp 1.0 83.4
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 679-694 bp 1.0 83.4
trnH-GUG-psbA_p5 GATCCACAATCCACTGCCTT GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 684-699 bp 1.0 83.4