Hotspot

rpl16

LSC · 79359-80781 · 1423 bp

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Pi 0.0178
MarkerSeek score 58.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1423
variable_sites
25
pi
0.0178
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 GTCTCAATTCCCGTGGGATC GTATGGAGGGAATTGCACGT 55.0 50.0 58.0 58.0 2528-2532 bp 1.0 81.0
rpl16_p2 ATCGGCGATTACTAGCTCCT GTATGGAGGGAATTGCACGT 50.0 50.0 58.1 58.0 2300-2304 bp 1.0 80.7
rpl16_p3 GTCTCAATTCCCGTGGGATC TGCACGTGTTGAATGGATCA 55.0 45.0 58.0 58.1 2176-2180 bp 1.0 80.6
rpl16_p4 ATTGTCTCAATTCCCGTGGG GTATGGAGGGAATTGCACGT 50.0 50.0 57.9 58.0 2531-2535 bp 1.0 80.3
rpl16_p5 ATCGGCGATTACTAGCTCCT TGCACGTGTTGAATGGATCA 50.0 45.0 58.1 58.1 1948-1952 bp 1.0 80.3