Hotspot

ndhE-ndhG

SSC · 116193-116464 · 272 bp

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Pi 0.0193

MarkerSeek score 63.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 272
variable_sites: 5
pi: 0.0193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GCTCAAGACACATAAGAGCCC	TGCTTTGGTAGGGGCAATTT	52.4	45.0	58.7	58.0	403-415 bp	1.0	69.6
ndhE-ndhG_p2	GCTCAAGACACATAAGAGCCC	TTGCTTTGGTAGGGGCAATT	52.4	45.0	58.7	58.0	404-416 bp	1.0	69.6
ndhE-ndhG_p3	GCTCAAGACACATAAGAGCC	TGCTTTGGTAGGGGCAATTT	50.0	45.0	56.2	58.0	403-415 bp	1.0	69.2
ndhE-ndhG_p4	GCTCAAGACACATAAGAGCC	TTGCTTTGGTAGGGGCAATT	50.0	45.0	56.2	58.0	404-416 bp	1.0	69.2
ndhE-ndhG_p5	GCTCAAGACACATAAGAGCCC	TTAGTTGCTTTGGTAGGGGC	52.4	50.0	58.7	57.8	408-420 bp	1.0	68.1