Hotspot

psbE-petL

LSC · 62540-63777 · 1238 bp

Back to Lobularia

Pi 0.0154

MarkerSeek score 67.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 19
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1384-1389 bp	1.0	67.1
psbE-petL_p2	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1385-1390 bp	1.0	66.5
psbE-petL_p3	ATAACCAGCCCGCAATGAAT	AAGCTGCTAGTAGAAAACCGA	45.0	42.9	57.9	57.0	1385-1390 bp	1.0	65.2
psbE-petL_p4	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	45.0	42.9	57.9	57.0	1384-1389 bp	1.0	65.2
psbE-petL_p5	CCGCAATGAATAGGGAAGGT	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.7	56.3	1375-1380 bp	1.0	64.9