Hotspot

petA-psbJ

LSC · 60807-61758 · 952 bp

Back to Lobularia

Pi 0.0222

MarkerSeek score 67.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 21
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.0	58.1	1140-1146 bp	1.0	73.0
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.0	57.8	1141-1147 bp	1.0	72.2
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	1137-1143 bp	1.0	71.9
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.2	57.8	1138-1144 bp	1.0	71.1
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1135-1141 bp	1.0	70.5