Hotspot

trnL-UAA-trnF-GAA

LSC · 46013-46372 · 360 bp

Back to Lobularia

Pi 0.0227

MarkerSeek score 54.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 8
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	CCTGGTTTACGCGAACAAAC	TACAGACCCTCGCGGAATAT	50.0	50.0	58.0	58.0	1230-1240 bp	1.0	81.6
trnL-UAA-trnF-GAA_p2	TGAATTCCAAGGTTTCGGCA	TACAGACCCTCGCGGAATAT	45.0	50.0	58.0	58.0	1429-1439 bp	1.0	81.5
trnL-UAA-trnF-GAA_p3	CCTGGTTTACGCGAACAAAC	CACGGCTGAAACGGATCTTA	50.0	50.0	58.0	58.0	1362-1372 bp	1.0	81.5
trnL-UAA-trnF-GAA_p4	TGAATTCCAAGGTTTCGGCA	CACGGCTGAAACGGATCTTA	45.0	50.0	58.0	58.0	1561-1571 bp	1.0	81.4
trnL-UAA-trnF-GAA_p5	TGAATTCCAAGGTTTCGGCA	AAAGCCGAGCCAATAGGATG	45.0	50.0	58.0	58.0	1305-1315 bp	1.0	81.3