Hotspot

ycf3-trnS-GGA

LSC · 43108-43347 · 240 bp

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Pi 0.0208

MarkerSeek score 64.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 5
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTCTCCGGAAGTTGTCGG	TGTTACGCCTTCAACCACTC	50.0	50.0	58.1	58.1	370 bp	1.0	79.1
ycf3-trnS-GGA_p2	TTTTCTCCGGAAGTTGTCGG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	426 bp	1.0	78.7
ycf3-trnS-GGA_p3	TGCCTCTTTTTCTCCGGAAG	TGTTACGCCTTCAACCACTC	50.0	50.0	57.8	58.1	377 bp	1.0	78.6
ycf3-trnS-GGA_p4	GCCTCTTTTTCTCCGGAAGT	TGTTACGCCTTCAACCACTC	50.0	50.0	57.8	58.1	376 bp	1.0	78.6
ycf3-trnS-GGA_p5	TGCCTCTTTTTCTCCGGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	433 bp	1.0	78.2