Hotspot

trnR-UCU-atpA

LSC · 8971-9044 · 74 bp

Back to Lobularia

Pi 0.0441

MarkerSeek score 74.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 74
variable_sites: 3
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GCGAAAAGCGTCCATTGTC	TAACCCCTGAAGCAGAAAGC	52.6	50.0	58.0	57.8	227-233 bp	1.0	73.5
trnR-UCU-atpA_p2	GCGAAAAGCGTCCATTGTC	AAGAGCAACTGGAGCGTTTT	52.6	45.0	58.0	58.3	188-194 bp	1.0	73.2
trnR-UCU-atpA_p3	GCGAAAAGCGTCCATTGTC	AGCAACTGGAGCGTTTTCTA	52.6	45.0	58.0	57.5	185-191 bp	1.0	71.5
trnR-UCU-atpA_p4	GCGAAAAGCGTCCATTGTC	ATTCAAGAGCAACTGGAGCG	52.6	50.0	58.0	58.6	192-198 bp	1.0	71.3
trnR-UCU-atpA_p5	GCGAAAAGCGTCCATTGTC	GCAACTGGAGCGTTTTCTAC	52.6	50.0	58.0	57.4	184-190 bp	1.0	71.1