Hotspot

trnS-GCU-trnG

LSC · 7284-7935 · 652 bp

Back to Lobularia

Pi 0.0154

MarkerSeek score 64.30

Primer available yes

Label trnS-GCU-trnG

Feature Metadata

label_name: trnS-GCU-trnG
feature_type: igs
region: LSC
length_bp: 652
variable_sites: 10
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG_p1	CCAACGCTTTAGTCCACTCA	AGGGGTTAAAGGAACGAATCA	50.0	42.9	57.8	56.9	727-730 bp	1.0	63.6
trnS-GCU-trnG_p2	CCAACGCTTTAGTCCACTCA	GGGGTTAAAGGAACGAATCAC	50.0	47.6	57.8	56.9	726-729 bp	1.0	63.3
trnS-GCU-trnG_p3	AACGCTTTAGTCCACTCAGC	AGGGGTTAAAGGAACGAATCA	50.0	42.9	58.2	56.9	725-728 bp	1.0	63.3
trnS-GCU-trnG_p4	AACGCTTTAGTCCACTCAGC	GGGGTTAAAGGAACGAATCAC	50.0	47.6	58.2	56.9	724-727 bp	1.0	63.0
trnS-GCU-trnG_p5	CCAACGCTTTAGTCCACTCA	AACCGAGAGACCCTTTAACT	50.0	45.0	57.8	55.8	752-755 bp	1.0	62.5