Hotspot

psbI-trnS-GCU

LSC · 7105-7195 · 91 bp

Back to Lobularia

Pi 0.0471

MarkerSeek score 79.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 4
pi: 0.0471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TCCTATCTAATGATCCAGGACGT	TAGGAGAGATGGCTGAGTGG	43.5	55.0	58.0	57.9	219-225 bp	1.0	59.0
psbI-trnS-GCU_p2	TCCTATCTAATGATCCAGGACGT	TGAGTGGACTAAAGCGTTGG	43.5	50.0	58.0	57.8	206-212 bp	1.0	58.5
psbI-trnS-GCU_p3	TCCTATCTAATGATCCAGGACGT	GCTGAGTGGACTAAAGCGTT	43.5	50.0	58.0	58.2	208-214 bp	1.0	58.2
psbI-trnS-GCU_p4	TCCTATCTAATGATCCAGGACGT	CGAGTTAATCGTACCGAGGG	43.5	55.0	58.0	57.6	169-175 bp	1.0	57.5
psbI-trnS-GCU_p5	TCCTATCTAATGATCCAGGACGT	ATGGCTGAGTGGACTAAAGC	43.5	50.0	58.0	57.6	211-217 bp	1.0	56.5