Hotspot

trnK-UUU-trnQ-UUG

LSC · 4334-5971 · 1638 bp

Back to Lobularia

Pi 0.0295

MarkerSeek score 74.30

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1638
variable_sites: 48
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AACTCTACCGCAGATCTGGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1764-1785 bp	1.0	80.9
trnK-UUU-trnQ-UUG_p2	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1696-1717 bp	1.0	80.9
trnK-UUU-trnQ-UUG_p3	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1695-1716 bp	1.0	80.9
trnK-UUU-trnQ-UUG_p4	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1682-1703 bp	1.0	78.5
trnK-UUU-trnQ-UUG_p5	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	1707-1728 bp	1.0	77.6