Hotspot

ORF304

LSC · 32803-33717 · 915 bp

Back to Lobelia

Pi 0.123

MarkerSeek score 82.60

Primer available yes

Label ORF304

Feature Metadata

label_name: ORF304
feature_type: gene
region: LSC
length_bp: 915
variable_sites: 743
pi: 0.123
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF304_p1	ACGAGTCCTGCGGATACATA	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	2079-2583 bp	0.888889	87.2
ORF304_p2	CCTGATCGATGAACCTGCAA	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	1907-2841 bp	0.822222	84.5
ORF304_p3	AACGTTACCGGCAATCTACC	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	1571-2006 bp	0.755556	82.0
ORF304_p4	AACGTTACCGGCAATCTACC	CTCACCGAAGACTGTTGAGG	50.0	55.0	58.0	57.9	1549-1984 bp	0.755556	81.5
ORF304_p5	AACGTTACCGGCAATCTACC	CACCTATTACCGCACCTGAC	50.0	55.0	58.0	58.1	1226-1336 bp	0.355556	63.6