Hotspot

ORF227

LSC · 56618-57301 · 684 bp

Back to Lobelia

Pi 0.0955

MarkerSeek score 79.50

Primer available yes

Label ORF227

Feature Metadata

label_name: ORF227
feature_type: gene
region: LSC
length_bp: 684
variable_sites: 440
pi: 0.0955
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF227_p1	AAAGCAGAAGCATAGACGCA	TTTTCGACGGAAGCTGCTTA	45.0	45.0	57.9	57.8	1166-1636 bp	0.911111	86.4
ORF227_p2	CTAGAAAGTTTCTCCGGCCC	TTTTCGACGGAAGCTGCTTA	55.0	45.0	58.0	57.8	1281-1751 bp	0.888889	86.1
ORF227_p3	CTAGAAAGTTTCTCCGGCCC	TCCTTCGTACGACCCCATTA	55.0	50.0	58.0	57.9	1654-1681 bp	0.355556	56.3
ORF227_p4	AAAGCAGAAGCATAGACGCA	TCCTTCGTACGACCCCATTA	45.0	50.0	57.9	57.9	1539-1566 bp	0.355556	55.8
ORF227_p5	TGCTAATGGGGGCTAAGACT	TCCTTCGTACGACCCCATTA	50.0	50.0	58.1	57.9	1632-1636 bp	0.333333	54.0