Hotspot

rpl32-ndhF

SSC · 123561-124635 · 1075 bp

Back to Lobelia

Pi 0.0433

MarkerSeek score 80.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1075
variable_sites: 455
pi: 0.0433
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGCCGATGAATATCCCTTCC	GTTGCCGACGGAAAAAGAAG	55.0	50.0	58.3	58.0	995-1359 bp	0.933333	86.9
rpl32-ndhF_p2	CGCCGATGAATATCCCTTCC	AGAAGTCCCACCCCGATTAA	55.0	50.0	58.3	58.0	977-1341 bp	0.933333	86.8
rpl32-ndhF_p3	CGCCGATGAATATCCCTTCC	TGCCGACGGAAAAAGAAGAA	55.0	45.0	58.3	58.0	993-1357 bp	0.933333	86.8
rpl32-ndhF_p4	CGCCGATGAATATCCCTTCC	AAGAAGTCCCACCCCGATTA	55.0	50.0	58.3	58.0	990-1342 bp	0.911111	85.9
rpl32-ndhF_p5	CGCCGATGAATATCCCTTCC	GCAGATTTCGAAACAGCACC	55.0	50.0	58.3	58.0	1622-1986 bp	0.866667	84.2