Hotspot

trnL (UAG)-rpl32

SSC · 122680-123395 · 716 bp

Back to Lobelia

Pi 0.0512
MarkerSeek score 81.00
Primer available yes
Label trnL (UAG)-rpl32

Feature Metadata

label_name
trnL (UAG)-rpl32
feature_type
igs
region
SSC
length_bp
716
variable_sites
324
pi
0.0512
unique_haplotype_count
37
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL (UAG)-rpl32_p1 AGCGTGTCTACCGATTTCAC ACATGGAAATGCAGTGTCGA 50.0 45.0 58.0 57.8 1038-1303 bp 0.844444 84.2
trnL (UAG)-rpl32_p2 CAGCGTGTCTACCGATTTCA ACATGGAAATGCAGTGTCGA 50.0 45.0 58.0 57.8 1039-1304 bp 0.844444 84.2
trnL (UAG)-rpl32_p3 AAAAGGTCGGTATCATGCCC ACATGGAAATGCAGTGTCGA 50.0 45.0 57.9 57.8 1547-1609 bp 0.755556 80.3
trnL (UAG)-rpl32_p4 GGTCGGTATCATGCCCTTTT ACATGGAAATGCAGTGTCGA 50.0 45.0 57.9 57.8 1543-1605 bp 0.755556 80.3
trnL (UAG)-rpl32_p5 AAAGACTAGAAGTGCAGCGG ACATGGAAATGCAGTGTCGA 50.0 45.0 57.9 57.8 1875-1936 bp 0.755556 80.1