Hotspot

rpl16-rps3

LSC · 80602-80766 · 165 bp

Back to Lobelia

Pi 0.0519

MarkerSeek score 78.80

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 61
pi: 0.0519
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TGCGAAGTGATGAATTGGGT	CGAGAAGGTAGAGTTCCCCT	45.0	55.0	57.8	57.9	376-399 bp	0.822222	82.1
rpl16-rps3_p2	AACGAGTCACACACTAAGCA	CGAGAAGGTAGAGTTCCCCT	45.0	55.0	57.1	57.9	296-318 bp	0.911111	80.2
rpl16-rps3_p3	TGCGAAGTGATGAATTGGGT	CCCTACAAACCATTCAAGCG	45.0	50.0	57.8	57.1	360-383 bp	0.888889	78.6
rpl16-rps3_p4	ACGAGTCACACACTAAGCAT	CGAGAAGGTAGAGTTCCCCT	45.0	55.0	56.9	57.9	295-317 bp	0.911111	78.4
rpl16-rps3_p5	CAACGAGTCACACACTAAGC	CGAGAAGGTAGAGTTCCCCT	50.0	55.0	56.8	57.9	297-319 bp	0.911111	77.6