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psbE-petL

LSC · 62722-63653 · 932 bp

Back to Lobelia

Pi 0.0191

MarkerSeek score 76.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 216
pi: 0.0191
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	677-1355 bp	1.0	90.7
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	679-1357 bp	1.0	90.7
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	763-1441 bp	1.0	90.6
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.2	58.0	765-1443 bp	1.0	90.6
psbE-petL_p5	TGCTGACGAATAACCAACCC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.2	58.0	1060-1446 bp	0.955556	88.7