Hotspot

ndhC-ORF227

LSC · 56165-56617 · 453 bp

Back to Lobelia

Pi 0.0654

MarkerSeek score 75.90

Primer available yes

Label ndhC-ORF227

Feature Metadata

label_name: ndhC-ORF227
feature_type: igs
region: LSC
length_bp: 453
variable_sites: 235
pi: 0.0654
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-ORF227_p1	CTAGAAAGTTTCTCCGGCCC	CAATATCCAAGCTCCGCAGT	55.0	50.0	58.0	58.0	906-1227 bp	0.866667	86.1
ndhC-ORF227_p2	CTAGAAAGTTTCTCCGGCCC	TTTTCTTGGTGGAAGGCCTC	55.0	50.0	58.0	58.0	1145-1235 bp	0.844444	85.5
ndhC-ORF227_p3	TCTCACTAAAGAAGGGGCCA	TTTTCTTGGTGGAAGGCCTC	50.0	50.0	58.0	58.0	1703-1793 bp	0.844444	85.4
ndhC-ORF227_p4	CTAGAAAGTTTCTCCGGCCC	CAAGACCCCCGTTTAACACA	55.0	50.0	58.0	58.0	515-833 bp	0.777778	82.7
ndhC-ORF227_p5	CTAGAAAGTTTCTCCGGCCC	ATCGATTAAGTTGCCGCCTT	55.0	45.0	58.0	58.0	1050-1093 bp	0.755556	81.2