Hotspot

psbM-trnD (GUC)

LSC · 35204-36216 · 1013 bp

Back to Lobelia

Pi 0.0234

MarkerSeek score 75.90

Primer available yes

Label psbM-trnD (GUC)

Feature Metadata

label_name: psbM-trnD (GUC)
feature_type: igs
region: LSC
length_bp: 1013
variable_sites: 264
pi: 0.0234
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD (GUC)_p1	TGCCAGAATATTGACTTCCA	AGATCCCTTAGTTCCCTGGG	40.0	55.0	54.3	57.8	898-1138 bp	0.977778	60.1
psbM-trnD (GUC)_p2	TGCCAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	40.0	50.0	54.3	58.5	871-1111 bp	1.0	58.8
psbM-trnD (GUC)_p3	ATGCCAGAATATTGACTTCCA	AGATCCCTTAGTTCCCTGGG	38.1	55.0	55.0	57.8	899-1139 bp	0.977778	57.2
psbM-trnD (GUC)_p4	TGCCAGAATATTGACTTCCAT	AGATCCCTTAGTTCCCTGGG	38.1	55.0	55.0	57.8	898-1138 bp	0.977778	57.2
psbM-trnD (GUC)_p5	GGAACTAAAATGAAGAGTGCAGT	AGATCCCTTAGTTCCCTGGG	39.1	55.0	57.1	57.8	930-1170 bp	0.955556	57.0