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ORF304-trnC (GCA)

LSC · 33718-34183 · 466 bp

Back to Lobelia

Pi 0.0848

MarkerSeek score 75.70

Primer available yes

Label ORF304-trnC (GCA)

Feature Metadata

label_name: ORF304-trnC (GCA)
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 273
pi: 0.0848
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF304-trnC (GCA)_p1	ATAGAGTCCAACCCCAACGA	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.1	58.0	768-915 bp	0.822222	84.3
ORF304-trnC (GCA)_p2	TACCCTCGCGACAAGATAGT	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	1440-1602 bp	0.733333	80.8
ORF304-trnC (GCA)_p3	TGTCCTTGTTATCCGAGGGT	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.1	58.0	924-1054 bp	0.577778	74.4
ORF304-trnC (GCA)_p4	TTCTTTACCCTCGCGACAAG	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	57.9	58.0	1465-1597 bp	0.288889	58.9
ORF304-trnC (GCA)_p5	TTTCGGTAGATGCAGAAGCC	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	880-905 bp	0.066667	45.7