Hotspot

atpH-atpI

LSC · 19219-20013 · 795 bp

Back to Lobelia

Pi 0.0302

MarkerSeek score 75.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 240
pi: 0.0302
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	918-959 bp	1.0	89.6
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	57.9	58.3	934-975 bp	1.0	88.7
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.2	58.3	919-960 bp	1.0	88.5
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	933-974 bp	1.0	88.2
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.3	58.3	928-969 bp	1.0	87.8