Hotspot

rbcL-atpB

LSC · 3941-4757 · 817 bp

Back to Lobelia

Pi 0.0272

MarkerSeek score 80.80

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 231
pi: 0.0272
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	ACACCAGCTTTGAATCCAACA	CAATGATTTGGCCGATACGC	42.9	50.0	58.3	58.0	860-956 bp	1.0	81.0
rbcL-atpB_p2	AGTCTCTGTTTGTGGTGACA	CAATGATTTGGCCGATACGC	45.0	50.0	56.6	58.0	831-927 bp	1.0	80.7
rbcL-atpB_p3	ACACCAGCTTTGAATCCAACA	CCCCAGAACCAGAAGGAGTA	42.9	55.0	58.3	58.0	812-908 bp	0.977778	80.1
rbcL-atpB_p4	ACACCAGCTTTGAATCCAACA	AGCTACATCTAGGACCGGAC	42.9	55.0	58.3	58.0	880-976 bp	0.911111	77.6
rbcL-atpB_p5	AGTCTCTGTTTGTGGTGACA	AGCTACATCTAGGACCGGAC	45.0	55.0	56.6	58.0	851-947 bp	0.911111	77.3