Hotspot

psbA

LSC · 91-1152 · 1062 bp

Back to Littledalea

Pi 0

MarkerSeek score 31.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTAGAACAAGATATTGGGCA	AATACGTGTGCTTGGGAGTC	40.9	50.0	57.5	57.9	1183 bp	1.0	59.2
psbA_p2	TGGGCATTTCTGTCTTTTCT	AATACGTGTGCTTGGGAGTC	40.0	50.0	55.1	57.9	1167 bp	1.0	56.3
psbA_p3	TGGGCATTTCTGTCTTTTCTT	AATACGTGTGCTTGGGAGTC	38.1	50.0	56.0	57.9	1167 bp	1.0	54.9
psbA_p4	TTGGGCATTTCTGTCTTTTCT	AATACGTGTGCTTGGGAGTC	38.1	50.0	56.0	57.9	1168 bp	1.0	54.9
psbA_p5	TGCTAGAACAAGATATTGGGCAT	AATACGTGTGCTTGGGAGTC	39.1	50.0	57.9	57.9	1183 bp	1.0	54.8