Hotspot

clpP

LSC · 68005-68655 · 651 bp

Back to Littledalea

Pi 0

MarkerSeek score 33.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 651
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CCAGGAAAACCCGAATTTAGC	ACTGCGGATTCTTTCTTTCTCT	47.6	40.9	57.5	57.7	789 bp	1.0	49.7
clpP_p2	CCAGGAAAACCCGAATTTAGC	GCGGATTCTTTCTTTCTCTTCC	47.6	45.5	57.5	57.4	786 bp	1.0	47.1
clpP_p3	CCAGGAAAACCCGAATTTAGC	ACTGCGGATTCTTTCTTTCTC	47.6	42.9	57.5	56.3	789 bp	1.0	46.1
clpP_p4	CAGGAAAACCCGAATTTAGCTG	ACTGCGGATTCTTTCTTTCTCT	45.5	40.9	57.9	57.7	788 bp	1.0	45.0
clpP_p5	GGAAAACCCGAATTTAGCTGTG	ACTGCGGATTCTTTCTTTCTCT	45.5	40.9	58.2	57.7	786 bp	1.0	44.4