Hotspot

rpl32-trnL-UAG

SSC · 105890-106613 · 724 bp

Back to Littledalea

Pi 0.00414

MarkerSeek score 47.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 724
variable_sites: 6
pi: 0.00414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CATTTTCCAGCGGCAATGAG	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.1	58.0	855-856 bp	1.0	80.0
rpl32-trnL-UAG_p2	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	823-824 bp	1.0	79.6
rpl32-trnL-UAG_p3	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	822-823 bp	1.0	79.6
rpl32-trnL-UAG_p4	CATTTTCCAGCGGCAATGAG	GGATTCGAACCGAGATGCTT	50.0	50.0	58.1	58.1	858-859 bp	1.0	79.5
rpl32-trnL-UAG_p5	AGATCATTTTCCAGCGGCAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.2	58.0	859-860 bp	1.0	79.3